CAS: 9002-07-7
This product is a biochemical grade genetically engineered enzyme, does not contain enzyme inhibitors such as DFP, PMSF and TLCK, and has the same activity and specificity as native Trypsin.The activity of this product is inhibited by serine protease inhibitors such as PMSF and TLCK, metal ion chelation agents such as EDTA
| Product Name | Recombinant Trypsin （Pharmacopoeia Grade） |
| Appearances | White or off-white powder |
| Enzyme Specific Activity | ≥3800USP unit/mg pro |
| Molecular Weight | 23.5kD±2.4kD |
| SDS-PAGE | ≥95% |
| HPLC Purity | α-Trypsin should NMT 20% ; β-Trypsin should NLT 70% |
| Protein Content | 40%~70% |
| Residue DNA | ≤100ng/mg pro |
| Residue HCP | ≤100ng/mg pro |
| Optimum pH | 7.0~9.0 |
| Solubility | Soluble in water or buffer |
| Storage Conditions | -15°C below, protected from light, sealed. |
The data should be subject to test report of products.
For biological medicine production, such as Insulin, GLP-1 drugs.
Protein structure and function studies, such as protein mass spectrometry, sequencing, peptide mapping analysis.
Protein preparation: The protein was dissolved in a solution of 25 mM Tris-HCl, pH 8.0.
Enzyme preparation: The desired enzyme powder was weighed and dissolved in 10 mM HCl to give an enzyme solution concentration of 1-10 mg/ml.
Reaction conditions The amount of enzyme: the target protein (mass ratio) = 1:50~1:1000, the optimum pH is 7.0~9.0.1.
Solution preparation:
Recombinant Trypsin is thawing at room temperature. According to the protein content in COA, take an appropriate amount of trypsin and add HBSS balance solution (or other buffers suitable for cell digestion, if you need to add EDTA, the final concentration is recommended to be 0-1mM, preferably no more than 2mM) The concentration of trypsin used is about 0.1- 0.3mg/ml (the concentration is adjusted according to different cells), and mix gently; this step can be performed at room temperature;
2. Use 0.22 μm filters to filter the above trypsin solution and transfer it to a sterile container; this step can be performed at room temperature;
3. The filtered trypsin solution should be used directly on the day as required (for example, add 1ml to a T25 flask, digest cells at 37°C)
Transportation stability: It is transported in an ice pack to keep it active.
Storage stability: The lyophilized powder can be stored for at least 24 months at -15°C below.
1 mM hydrochloric acid or 50 mM acetic acid was dissolved and stored at -15°C below, repeated freeze- thaw 10 times, no loss of activity.
It is recommended that the storage should not exceed 7 days after dissolution at 2~8℃, and it should not exceed 30 days after storage at -15°C below.
If the temperature exceeds 37 °C, this product may self-digest.
The product has moisture absorption and should be taken after room temperature balance.
The product is for research use only and should not be used for diagnosis or treatment of animals or humans.
