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Recombinant Carboxypeptidase B(with His Tag)

CAS: 9025-24-5


Recombinant Carboxypeptidase B (with His Tag) is a lyophilized powder that is isolated and purified from recombinant Pichia pastoris and then freeze-dried, and has the same enzyme activity and specificity as natural carboxypeptidase B.

Recombinant Carboxypeptidase B(with His Tag)

Recombinant Carboxypeptidase B (with His Tag)Specifications

Product NameRecombinant Lysyl Endopeptidase (Biochemical Grade)
AppearancesWhite or off-white powder
Specific Activity≥80U/mg pro
MW36kD±3.6kD
Purity≥95%(SDS-PAGE)
Protein Content20%-60%
Residue DNA≤100ng/mg pro
Residue HCP≤100ng/mg pro
Optimum pH7.0~9.0
SolubilitySoluble in buffer
Storage Conditions-15°C below, protected from light,sealed.

The data should be subject to test report of products.



Carboxypeptidase B Application

  • Production of various proteins or polypeptides, such as insulin and analogs, cosmetic peptides, antimicrobial peptides, food flavor peptides, and the like.


  • Quality testing of antibodies. 


  • Determination of the C-terminal amino acid of a protein



Carboxypeptidase B Recommended Method

  • Protein preparation: The protein was dissolved in a solution of 25 mM Tris-HCl, pH 7.6.


  • Enzyme preparation: The desired enzyme powder was weighed and dissolved in 25 mM Tris-HCl (pH 7.6) to give an enzyme solution concentration of 1~10 mg/ml.


  • Reaction recommended enzyme amount: target protein (mass ratio) = 1:50 ~ 1:1000, optimum pH is 7.0~9.0.

Carboxypeptidase B



Carboxypeptidase B Transport and Storage Stability

  • Transportation stability: It is transported in an ice pack to keep it active.


  • Storage stability: The lyophilized powder can be stored for at least 24 months at -15°C below.


  • 25 mM Tris-HCl (pH 7.6) was dissolved and stored at -15°C below, and repeatedly frozen and thawed 10 times without loss of activity.


  • It is recommended not to exceed 7 days after storage at 2~8°C, and no more than 30 days for -15°C below.



Notice and Disclaimer

  • Activity is competitively inhibited by arginine and lysine, and metal ion chelating agents such as EDTA also inhibit enzyme activity.


  • The product is hygroscopic and should be taken after equilibration at room temperature.


  • The product is for research use only and should not be used for diagnosis or treatment of animals or humans.

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All products from the site for Research Use Only. Not for use in diagnostic procedures. Reproduction of any materials is strictly forbidden without permission.