CAS.NO: 62253-63-8
The recombinant human epidermal growth factor (EGF) is a biologically active protein factor for cell culture, a type of polypeptide substance widely present in mammals, which exerts a physiological action by binding to the EGF receptor on the cell membrane.
| Product Name | Recombinant Human Epidermal Growth Factor |
| Species | Human |
| Source | Pichia pastoris |
| Appearances | White or off-white lyophilized powder |
| Molecular weight | 6.0kD±0.6 kD |
| Purity | ≥95%（SDS-PAGE） ≥95%（RP-HPLC） |
| Endotoxin | < 0.2 EU/µg |
| Active | ≥1.2×106U/mg pro |
| Solubility | Good solubility in water, buffer, and medium |
| Storage Conditions | -15℃ below, protected from light, sealed |
The data should be subject to test report of products.
Recombinant human EGF is widely used in cell culture of fibroblasts, keratinocytes, epithelial cells and endothelial cells.
It is recommended to centrifuge the powder to the bottom of the tube before opening the lid, then dissolve it in sterilized deionized water to prepare a storage solution of 100 μg/ml. The recombinant human EGF can be stored stably for 1 week at 2~ 8 ℃ . When diluted to a lower concentration (not less than 10μg/ml), 0.1% tissue culture grade BSA or HSA is required. If it is necessary to extend the storage time, it is recommended to freeze at -70 ℃ or -20 ℃ rather than multigelation. The EGF epidermal growth factor should be determined by experiment to determine the optimum concentration according to each specific application.
Transportation stability: Our recombinant human EGF is transported in an ice pack to keep it active.
Storage stability: stable to 24 months at -15°C below, stable to 4 weeks at 2~8°C. Prepared as a storage solution of 100μg/ml, stable storage at 2~8°C for one week.
Avoid long-time storage in high-temperature environments.
Avoid contamination during the operation of our EGF epidermal growth factor.
The epidermal growth factor protein is for research use only and should not be used for the diagnosis or treatment of animals or humans.
