CAS: 99676-46-7
Gene-Biocon Recombinant Kex2 is a genetically engineered protein expressed in Pichia pastoris and purified by high-pressure liquid chromatography. The optimal pH of Kex2 protease is 9.0, and the optimal temperature is 37 ℃. It is stable in buffer (pH 5.0-6.0).
| Product Name | Recombinant Kex2 Protease |
| Appearances | White or off-white powder powder |
| Specific Activity | ≥10U/mg pro. |
| MW | 70.0 ± 7.0 kDa |
| Residue DNA | ≤100ng/mg pro. |
| Residue HCP | ≤100ng/mg pro. |
| Storage Conditions | -15°C below, protected from light, sealed. |
The data should be subject to test report of products.
Recommended reaction buffer: pH 7.0-9.0, 50mM Tris-HCl, 2mM Ca2+ or HEPES, 5mM Ca2+. If it is not used immediately after dissolution, it is recommended to use 20mM (pH 5.2) NaAc- HAc, 2mM Ca2+ buffer to dissolve the lyophilized powder.
During the reaction, use pH 7.0-9.0, 50mM Tris-HCl, 2mM Ca2+ or HEPES, 5mM Ca2+ as the reaction diluent.
Transport stability: It is transported in an ice pack to keep it active. Storage stability: Store at -15°C below, stable within 24 months. Dissolve in storage buffer (20mM NaAC-HAC, 2mM Ca2+, pH5.2) and store in sub-packages -15ºC below stable within 6 months.
For the specific optimal testing concentration, please refer to the relevant literature, or explore and optimize through experiments according to the purpose of the experiment
The product is for research use only and should not be used for diagnosis or treatment of animals or humans.
