CAS.NO: 72561-05-8
Recombinant Lysyl Endopeptidase (Biochemical Grade) is highly specific and specifically cleaves peptide bonds at the carboxy terminus of lysine residues and S- aminoethyl cysteine residues. It has also been reported to have a small amount of non-specific cleavage.
| Product Name | Recombinant Lysyl Endopeptidase (Biochemical Grade) |
| Appearances | White or off-white powder |
| Activity | ≥1.0AU/mg pro |
| MW | 36kD±3.6kD |
| Purity | Single main band (SDS-PAGE) with a molecular weight of 27.7 kD ± 2.8 kD（SDS-PAGE） |
| Protein Content | 30%-70% |
| Residue DNA | ≤100ng/mg pro |
| Residue HCP | ≤100ng/mg pro |
| Optimum pH | 8.5~9.5 |
| Solubility | Soluble in buff |
| Storage Conditions | -15°C below, protected from light，sealed. |
The data should be subject to test report of products.
Production of biological products, such as insulin and analog production, production of GLP-1 analogues.
Protein structure and function studies, such as protein mass spectrometry, sequencing, peptide mapping analysis.
Production of small molecular proteins or peptides, such as cosmetic peptides, antimicrobial peptides, food flavor peptides, and the like.
Enzymatic synthesis of Lys-X compounds.
The cultivation of stem cells and primary cells is more moderate.
Protein preparation
Enzyme preparation
Digestive response
Transport stability: The Lysyl Endopeptidase is transported in an ice pack to keep it active.
Storage stability: Store at -15°C below, stable within 24 months; Store at -15°C below with a solution of enzyme concentration greater than 1mg/ml in Tris- HCl buffer pH 8.0 to pH 11.0 at -15°C below, repeated freeze-thaw 10 times, no loss of activity. It is recommended not to exceed 7 days after storage at 2~8°C, and no more than 30 days for -15°C below.
The Lysyl Endopeptidase may self-digest when the temperature exceeds 37°C.
Most of the viability is retained in 1M urea or 0.1% SDS.
